Addressing the problem of hook effect in sandwich lateral flow immunoassay: review and impact on future solutions
10.3760/cma.j.cn114452-20230203-00068
- VernacularTitle:解决夹心式侧流免疫层析中钩效应的问题：回顾及对未来解决方案的影响
- Author:
Shang LIU
1
;
Yabing YE
;
Kangsong HE
;
Jianbin TANG
Author Information
1. 浙江大学化学工程与生物工程学院生物工程研究所生物与医药专业2021级，杭州310058
- Keywords:
Immunoassay;
Nanoparticles;
Gold colloid;
False negative reactions
- From:
Chinese Journal of Laboratory Medicine
2023;46(4):415-420
- CountryChina
- Language:Chinese
-
Abstract:
Lateral flow immunoassay (LFIA) is a rapid detection technique that allows researchers to move the antigen-antibody reaction from a test tube or laboratory vessel to a test strip. Due to the chromatographic effect of the test strip, the solution would move to a specified direction based on the test and complete the whole antigen-antibody specific reaction. A qualitative judgment can be made with the naked eye by observing the color change of the reagent strip at a specific location. Because of its advantages of being fast, simple, specific, inexpensive, and requiring no specialized personnel, LFIA is now widely used in medical testing, food quality monitoring, environmental monitoring, agriculture and animal husbandry. A major bottleneck for the development of LFIA technology is the hook effect. This paper summarizes the current methods, means and research progresses to combat the hook effect, hoping to provide a strong technical reference for researchers to design test strips, select suitable nanoparticles, and achieve quantitative LFIA detection.
