Nucleic acid detection and sequence analysis of arboviruses in Xichang
10.3760/cma.j.cn114452-20221011-00586
- VernacularTitle:西昌市虫媒病毒核酸检测及其序列分析
- Author:
Yiju CHEN
1
;
Yuwen HE
;
Yiling YANG
;
Jin SUN
;
Zhenxing YANG
;
Jinxin MENG
;
Nan LI
;
Chuanzhi XU
;
Jinglin WANG
Author Information
1. 昆明医科大学公共卫生学院云南省教育厅公共卫生与疾病防控重点实验室，昆明 650500
- Keywords:
Arboviruses;
Molecular diagnostic detection technology;
Heredity;
Culex
- From:
Chinese Journal of Laboratory Medicine
2023;46(2):143-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the prevalence of arboviruses in mosquito samples in Xichang City, Sichuan Province, and enrich the data of arbovirus activity and genetic characteristics in southwestern Sichuan Province.Methods:In June 2018, the nucleic acid was extracted from Culex tritaeniorhynchus mosquitoes collected from different pigsties in three villages and suburbs of Xichang City. The specific primers of Yunnan orbivirus, Banna virus, Tibet orbivirus (S7, S10), Flavivirus and alphavirus were used for quantitative polymerase chain reaction examination, and the positive product was cloned for sequencing analysis. Results:A total of 9 012 mosquitoes were collected, of which Cx. tritaeniorhynchus was the dominant species. A number of 88 batches of these mosquitoes were amplified, and 2 strains of Japanese encephalitis virus (JEV), 7 strains of Banna virus (BAV), 7 strains of Tibet orbivirus (TIBOV) and 1 strain of Yunnan orbivirus virus (YOUV) were detected, respectively. By the results of cluster analysis and evolutionary tree analysis, the 17 newly found virus strains were close to the Yunnan isolates, and 2 JEV strains were located in the GI-b clade. The other 7 strains of BAV were A2 evolutionary clades. Of the 7 TIBOV plants, 6 were located in the same clade. One TOUV was in the same clade as the Yunnan strain. Conclusions:Culex tritaeniorhynchus mosquitoes in Xichang city might carry JEV, BAV, YOUV and TIBOV, among them JEV was GI-b type and BAV was A2 type. The results provide data supporting the detection and analysis of arboviruses in Xichang city.
