Advances in single-cell RNA sequencing in the retina
10.3760/cma.j.cn511434-20220117-00030
- VernacularTitle:视网膜单细胞转录组测序研究进展
- Author:
He ZHU
1
;
Xiangtian ZHOU
;
Fuxin ZHAO
Author Information
1. 温州医科大学附属眼视光医院 眼视光学和视觉科学国家重点实验室, 温州 325027
- Keywords:
Single-cell RNA sequencing;
Retina;
Retinal development;
Retinal degeneration;
Review
- From:
Chinese Journal of Ocular Fundus Diseases
2023;39(1):73-77
- CountryChina
- Language:Chinese
-
Abstract:
Retina is composed of a heterogeneous population of cell types, each with a unique biological function. Even if the same type of cells, due to genetic heterogeneity will lead to cell function differences. In the past, traditional molecular biological methods cannot resolve variations in their functional roles that arise from these differences, and some cells are difficult to define due to the lack of specific molecular markers or the scarcity of numbers, which hindered the understanding and research of these cells. With the development of biotechnology, single-cell RNA sequencing can analyze and resolve differences in single-cell transcriptome expression profiles, characterize intracellular population heterogeneity, identify new and rare cell subtypes, and more definitely define the characteristics of each cell type. It clarifies the origin, function, and variations in cell phenotypes. Other attributes include pinpointing both disease-related characteristics of cell subtypes and specific differential gene expression patterns, to deepen our understanding of the causes and progression of diseases, as well as to aid clinical diagnosis and targeted therapy.
