Progress in protein extraction methods for fungal identification with MALDI-TOF MS
10.3760/cma.j.cn112309-20230314-00058
- VernacularTitle:用于MALDI-TOF MS鉴定的真菌蛋白质提取方法的研究进展
- Author:
Jiayin WANG
1
;
Zeyu SUN
;
Jiancheng XU
Author Information
1. 吉林大学第一医院检验科，长春 130021
- Keywords:
MALDI-TOF MS;
Protein extraction methods;
Fungal cell wall;
Yeast-like fungi;
Filamentous fungi
- From:
Chinese Journal of Microbiology and Immunology
2023;43(8):647-652
- CountryChina
- Language:Chinese
-
Abstract:
With the development of techniques for rapid microbial identification, MALDI-TOF MS has become an important tool for clinical identification of fungi. Problems such as the applicability and standardization of protein extraction methods have hindered the development of MALDI-TOF MS technology in the fungal field. This paper analyzed the complex structure of fungal cell walls, introduced the protein extraction methods recommended by MALDI-TOF MS commercial mass spectrometry systems, discussed the protein extraction methods for the identification of various genera of yeast-like fungi and filamentous fungi by MALDI-TOF MS, such as direct smear method, formic acid acetonitrile extraction method and magnetic bead grinding method, and summarized the current status and drawbacks of protein extraction methods in fungal identification by MALDI-TOF MS with a view to providing theoretical reference for subsequent research.
