Epidemiological investigation and genomic characterization of the first case of fever with thrombocytopenia syndrome in Puyang
10.3760/cma.j.cn112309-20230211-00028
- VernacularTitle:濮阳市首例发热伴血小板减少综合征病例流行病学调查及基因组特征分析
- Author:
Zeqian CHEN
1
;
Yi LI
;
Xiaoyang WANG
;
Shujun PEI
;
Wenshuai SUO
;
Zhiquan HE
;
Aiguo YOU
;
Yibin HAO
;
Xueyong HUANG
Author Information
1. 郑州大学公共卫生学院流行病与卫生统计学教研室，郑州 450001
- Keywords:
Severe fever with thrombocytopenia syndrome;
Novel bunyavirus;
Gene sequencing;
Homology
- From:
Chinese Journal of Microbiology and Immunology
2023;43(8):627-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To report the first case of sever fever with thrombocytopenia syndrome caused by severe fever with thrombocytopenia syndrome virus (SFTSV) in Puyang city, and to study the epidemiological and molecular characteristics of S, M, L fragments of the SFTSV isolate.Methods:The epidemiological characteristics of this case was analyzed with epidemiological methods. SFTSV was isolated from the patient′s serum sample. Nucleic acid of SFTSV was extracted and detected by fluorescent RT-PCR. A multiplex PCR method was constructed to amplify the nucleic acid sequence of the virus. whole-genome sequencing was performed on the next-generation sequencing platform. MEGA11 and DNAStar was used for homology analysis and a phylogenetic tree was constructed.Results:Epidemiological investigation showed that the patient and his close contacts had no history of travel or tick bite within 14 d, but had a history of fieldwork. The patient′s serum sample was positive for SFTSV nucleic acid. Genetic analysis showed that the S, M, L gene fragments of the first SFTSV isolate in Puyang belonged to genotype E. This isolate shared 94.8%-99.6%, 94.0%-99.8% and 95.7%-99.7% nucleotide sequence homology with the representative strains acquired from GeneBank in S, M, L gene fragments, respectively.Conclusions:This case was the first case of SFTSV-caused severe fever with thrombocytopenia syndrome in Puyang. The SFTSV isolate shared a close homology with domestic isolates, but its genotype was significantly different from the SFTSV strains isolated in Henan in recent years, indicating that it might an imported case from other places in Henan Province or Hubei Province. Disease monitoring and professional training for medical personnel should be strengthened and more attention should be paid to the evolution and mutation of SFTSV.
