Structure and mechanism of Candida albicans Int1 involved in septin organization regulation
10.3760/cma.j.cn112309-20230214-00034
- VernacularTitle:白念珠菌anillin家族蛋白Int1调控septin细胞骨架组织的结构与功能研究
- Author:
Huan WU
1
;
Weina GUO
;
Qingjie MENG
;
Jun YAN
Author Information
1. 华中科技大学同济医学院附属武汉儿童医院检验部，武汉 430016
- Keywords:
Candida albicans;
Int1;
Septin;
Hyphae;
Cytoskeleton
- From:
Chinese Journal of Microbiology and Immunology
2023;43(6):425-431
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of Candida albicans Int1 in regulating septin organization. Methods:A series of full-length and truncated fragments of Int1 were constructed and fused with green fluorescent protein (GFP). The intracellular localization of the fusion proteins was observed under a fluorescence microscope. The region in Int1 that was required for bud neck localization was identified. Full-length and fragments of Int1 were overexpressed in the yeast Saccharomyces cerevisiae and the changes in cell growth, cell morphology and septin organization were investigated to determine the functional region in Int1 that mediated the interaction with septin. Moreover, the co-localization of the region and septin was analyzed. Results:The full-length Int1 consisted of 1 661 amino acid residues. A middle region of 209 amino acid residues, Int1-M4 (739-947 aa), that could be localized at the bud neck during both small and large bud periods was identified. Overexpression of Int1-M4 led to significant growth defects, elongated bud and disorganized septin. In the cells with elongated bud, Int1-M4 and septin with abnormal structures could be co-localized.Conclusions:Int1-M4 (739-947 aa), the middle region of Int1 containing 209 amino acid residues, mediated the bud neck localization and the interaction with septin, playing an important role in regulating septin organization.
