Immunogenicity of quadrivalent influenza virus subunit vaccine combined with RFH01 adjuvant in mice
10.3760/cma.j.cn112309-20221017-00336
- VernacularTitle:四价流感病毒亚单位疫苗配伍RFH01佐剂在小鼠体内的免疫原性研究
- Author:
Rong ZHOU
1
;
Ning MA
;
Guomei ZHANG
;
Yang LE
;
Xuanxuan NIAN
;
Xuedan LI
;
Lanxin JIA
;
Jiayou ZHANG
;
Yangyang ZHANG
;
Xiaoming YANG
Author Information
1. 武汉生物制品研究所有限责任公司病毒性疫苗研究二室，武汉 430207
- Keywords:
Influenza virus vaccine;
Adjuvant;
Subunit vaccine;
Immunogenicity
- From:
Chinese Journal of Microbiology and Immunology
2023;43(5):351-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the immunogenicity of a quadrivalent subunit vaccine combined with RFH01 adjuvant in a mouse model.Methods:Identification tests were performed on four monovalent influenza virus subunit vaccine stock solutions according to the methods described in Part 3 of the Chinese Pharmacopoeia 2020 Edition. In the study of the quadrivalent subunit vaccine combined with RFH01 adjuvant, 460 female BALB/c mice (6-8 weeks old) were randomly divided into 46 groups including experimental groups, vaccine control group, negative control group and blank group with 10 mice in each group. In the study of the quadrivalent subunit vaccine in old and young mice, 80 female 10-month-old and 80 female 10-week-old BALB/c mice were randomly divided into 16 groups ( n=10) including monovalent influenza virus vaccine group, quadrivalent subunit vaccine group, quadrivalent subunit vaccine+ RFH01 adjuvant group, chicken embryo quadrivalent split vaccine control group and PBS group. All mice were immunized by intramuscular injection. At 21 d after the primary immunization, a booster immunization was conducted using the same strategy. Blood samples were collected at 21 d and 42 d after the primary immunization for serum separation. Haemagglutination inhibition (HI) test was performed to detect the antibody levels in mouse serum samples. Results:After the booster immunization, the positive conversion rates in all vaccine+ RFH01 adjuvant groups reached 100%, and the geometric mean titers (GMTs) of serum antibodies were significantly higher than those of the vaccine groups without RFH01 adjuvant. There were significant differences in serum antibody titers between the monovalent/quadrivalent subunit vaccine groups with and without RFH01 adjuvant. After the booster immunization, the titers of serum antibodies against H1N1, H3N2, B/Victoria and B/Yamagata in the 10-week-old mice were significantly higher than those in the 10-month-old mice.Conclusions:The monovalent and quadrivalent influenza virus vaccines in combination with RFH01 adjuvant could elicit higher antibody titers in young (6-10 weeks old) and old (10 months old) mice, showing good immunogenicity.
