Characteristics of HIV-1 CRF01_AE molecular network in Yunnan Province
10.3760/cma.j.cn112309-20220913-00291
- VernacularTitle:云南省HIV-1 CRF01_AE毒株分子网络分析
- Author:
Rui ZHANG
1
;
Mengxue LI
;
Mi ZHANG
;
Jianjian LI
;
Xuemei DENG
;
Jiali WANG
;
Jiafa LIU
;
Xingqi DONG
Author Information
1. 云南省传染病医院，昆明医科大学附属传染病医院检验科，昆明 650301
- Keywords:
HIV-1;
CRF01_AE;
Molecular network
- From:
Chinese Journal of Microbiology and Immunology
2023;43(5):333-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular network of HIV-1 CRF01_AE in Yunnan Province and the factors influencing it.Methods:Demographic data and plasma samples of HIV/AIDS patients in Yunnan drug resistance monitoring database from 2018 to 2021 were collected. HIV-1 pol gene fragments (protease and reverse transcriptase region) were amplified using RT-PCR and then sequenced. The optimal gene distance was selected and a molecular network was constructed based on the sequences of CRF01_AE genotype. Results:In this study, a total of 967 sequences of CRF01_AE genotype were obtained by sequencing. At the optimal gene distance threshold of 1.75%, a total of 320 sequences were involved in the network with a rate of 33.1%, and 84 clusters were identified. In the regional distribution, one cluster dominated by multiple regions, one cluster dominated by Zhaotong, one cluster dominated by Honghe and five clusters dominated by Wenshan were formed in the network. In the network, 75.8% of heterosexual men were connected with other heterosexual men and 54.1% were connected with heterosexual women. There was potential transmission among 66.7% of men who have sex with men (MSM). HIV/AIDS patients in Chuxiong, Dali, Dehong, Honghe, Lincang, Pu′er, Wenshan, Yuxi and Zhaotong were more likely to be involved in the network that those in Kunming. People who were 50 years old and above were more likely to be involved in the network than those less than 25 years old. Factors influencing HIV/AIDS patients with HIV-1 CRF01_AE infection to become high-risk transmitters in Yunnan were not found and further study on this subject was needed.Conclusions:HIV-1 CRF01_AE strains had spread actively in different regions of Yunnan Province and the transmission network was complex. Dynamic monitoring of CRF01_AE strains should be strengthened and a precise intervention for high-risk transmitters should be performed to reduce new infections.
