Rabies virus viability in vitro
10.3760/cma.j.cn112309-20220926-00311
- VernacularTitle:狂犬病病毒体外生存活力研究
- Author:
Ranxin ZHANG
1
;
Zhengran LIU
;
Xuexin LU
;
Wuyang ZHU
Author Information
1. 包头医学院公共卫生学院，包头 014000
- Keywords:
Rabies virus;
Viability;
Disinfectant;
Virus isolation
- From:
Chinese Journal of Microbiology and Immunology
2023;43(3):203-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the in vitro viability of rabies virus in tissues and body fluid samples. Methods:The viability of rabies virus in tissues and suspensions was analyzed by virus titer determination method, direct immunofluorescence, RT-PCR and laboratory techniques for virus isolation.Results:With the increase of temperature, the viability of rabies virus in brain tissues and suspensions decreased gradually. Rabies virus lost infectivity after 30 min at 56℃, but remained viable in tissues for 7 d at 37℃. The virus showed no viability after 1 h at pH9.6. The rabies virus in suspensions could be completely inactivated after the stimulation with ethanol at a final concentration above 30%, sodium hypochlorite above 500 mg/L or benzalkonium bromide above 100 mg/L for 3 min. It was found that 80% acetone had the strongest inactivation effect on rabies virus in tissues, and no virus could be isolated after soaking for 4 h.Conclusions:Rabies virus was not tolerant to high temperature and relatively stable in the environment with pH6.8-7.4. Common disinfectants could kill the virus. This study provided detailed data about the viability of rabies virus in vitro, which would be conducive to the prevention and control of rabies.
