Isolation and genome-wide evolutionary analysis of a type Ⅲ WU polyomavirus strain
10.3760/cma.j.cn112309-20230116-00012
- VernacularTitle:一株Ⅲ型WU多瘤病毒的分离培养和全基因组进化分析
- Author:
Yiman HUANG
1
;
Aijun CHEN
;
Chao WANG
;
Tianli WEI
;
Zhuang HU
;
Shanshan CONG
;
Jingjing TAN
;
Lihong YAO
;
Lishu ZHENG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，国家卫生健康委员会医学病毒和病毒病重点实验室，北京 100052
- Keywords:
WU polyomavirus;
Virus isolation;
Human airway epithelial cell;
Whole-wide analysis
- From:
Chinese Journal of Microbiology and Immunology
2023;43(3):182-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and culture WU polyomavirus (WUPyV), and to analyze the genome-wide evolutionary patterns, homology and population dynamics.Methods:Real-time quantitative PCR was used to detect the nasopharyngeal aspirate samples of hospitalized children with respiratory tract infection in Beijing Friendship Hospital during 2020 to 2022. Primary human airway epithelial cells cultured at the air-liquid interface were used to isolate and culture WUPyV. Whole genome sequence of the isolated strain was obtained by Sanger sequencing. For phylogenetic and evolutionary dynamics analysis, the whole genome was compared with the published whole genome sequences in GenBank database.Results:The detection rate of WUPyV was 4.7% (31/659) during 2020 to 2022, and a clinical strain BJ0593 of WUPyV type Ⅲc was successfully isolated. The homology of the whole genome and gene fragments of WUPyV was high. The average evolutionary rate of VP2 gene was about 1.256×10 -4 substitution/site every year, and the population dynamics of WUPyV tended to be flat in the last decade. Conclusions:This study successfully isolated a clinical WUPyV type Ⅲ strain for the first time, which provided the basis for further investigation on the molecular evolution and pathogenicity of WUPyV.
