Immunoprotective role of dendritic cells in Chlamydia muridarum respiratory infection
10.3760/cma.j.cn112309-20220703-00223
- VernacularTitle:树突状细胞在沙眼衣原体小鼠肺炎株呼吸道感染中免疫保护作用的研究
- Author:
Ruoyuan SUN
1
;
Lu TAN
;
Xiaoyu ZHA
;
Yuqing TUO
;
Shuaini YANG
;
Jiajia ZENG
;
Yueyue XU
;
Hong ZHANG
;
Tongxing QU
;
Xuejun ZHANG
;
Hong BAI
Author Information
1. 天津医科大学基础医学院免疫学系，国家教育部免疫微环境与疾病重点实验室，天津市细胞和分子免疫学重点实验室，天津 300070
- Keywords:
Immunity;
Chlamydia muridarum;
Dendritic cell;
Th1 immune response
- From:
Chinese Journal of Microbiology and Immunology
2023;43(2):123-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of dendritic cells (DC) in Chlamydia muridarum ( Cm) respiratory infection and their effect on adaptive immune response. Methods:C57BL/6 mice were exposed to 1×10 3 inclusion-forming units (IFU) of Cm through inhalation to establish the mouse model of Cm respiratory infection. The proportion of CD11c + MHCⅡ + DC and the expression of costimulatory molecules (CD40, CD80 and CD86) in spleen tissues were detected by flow cytometry on 0, 3 and 7 d after infection. The expression of IL-12p40, IL-10 and IL-6 at mRNA level in spleen tissues was detected by qPCR. Mouse splenic DC isolated on 7 d after Cm infection were sorted by magnetic beads and then transferred to recipient mice. Th1 response in the recipient mice was measured using intracellular cytokine staining 14 d after infection. Results:Cm respiratory infection induced massive infiltration of DC and promoted the expression of costimulatory molecules on splenic DC. The expression of IL-12 and IL-10 at mRNA level in splenic DC reached the peak on 3 d after infection. Transferring the splenic DC of Cm-infected mice into the recipient mice could alleviate the disease condition in the recipient mice after Cm infection with reduced Cm inclusion-forming units in lung tissues and significantly increased proportion of Th1 cells in lung and spleen tissues. Conclusions:Cm respiratory infection could induce the maturation and activation of DC, which promoted Th1 immune response. DC played an important role in Cm infection.
