Gene expression profile in human cervical epithelial cells infected with Chlamydia trachomatis
10.3760/cma.j.cn112309-20220609-00194
- VernacularTitle:人正常宫颈上皮细胞感染沙眼衣原体的基因表达谱分析
- Author:
Ru JIA
1
;
Chenli SI
;
Mingyang LI
;
Jia YANG
;
Xinlei WU
;
Shanli ZHU
Author Information
1. 温州医科大学第一临床医学院（信息与工程学院），温州 325035
- Keywords:
Chlamydia trachomatis;
Human cervical epithelial cell;
Transcriptome;
Differentially expressed gene
- From:
Chinese Journal of Microbiology and Immunology
2023;43(2):93-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare gene expression profiles in normal human cervical epithelial cells (HcerEpic) before and after Chlamydia trachomatis ( Ct) infection. Methods:HcerEpic cells that were pretreated with DEAE-D were infected with Ct serotype E standard strain and then cultured for 44 h. Uninfected HcerEpic cells were used as the control group. Total RNA was extracted from the cells in each group and reverse transcribed to construct a cDNA library. Differences in gene expression profiles between the two groups were analyzed by high-throughput sequencing and the representative genes were selected for verification by qPCR. Results:A total of 23 997 genes were detected, including 125 differentially expressed genes. Among the 125 genes, 119 were up-regulated and six were down-regulated. GO analysis showed that the differentially expressed genes were enriched in several biological processes including defense response to virus, typeⅠinterferon signaling pathway and cellular responses to typeⅠinterferons. KEGG enrichment analysis showed the differentially expressed genes were mainly enriched in the pathways related to virus infections, such as influenza A virus, herpes simplex virus, EB virus and HPV, and NOD-like receptor pathway.Conclusions:There were significant differences in transcriptome profiles of HcerEpic cells before and after Ct infection. The differentially expressed genes were mainly enriched in the interferon pathway, which was closely related to the antiviral processes in cells. qPCR verified the differentially expressed genes and the genes closely related to the interferon pathway, such as ISG15, IFIT2, OASL and UBE2L6.
