Ultrashortwave therapy can inhibit inflammation and promote recovery from spinal cord injury
10.3760/cma.j.issn.0254-1424.2023.01.002
- VernacularTitle:超短波治疗对脊髓损伤炎症因子和丝裂原活化蛋白激酶通路的影响
- Author:
Shuai WANG
1
;
Jialin LIU
;
Li NA
;
Peng SU
;
Hongxuan DONG
;
Lin LI
;
Jian GAO
;
Lixin ZHANG
Author Information
1. 中国医科大学附属盛京医院康复中心，沈阳 110134
- Keywords:
Spinal cord injury;
Ultrashortwave therapy;
MAPK;
Inflammatory factors
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2023;45(1):6-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe any effect of ultrashortwave (USW) therapy on inflammatory cytokines and the MAPK pathway of rats with a spinal cord injury.Methods:Seventy-nine Sprague-Dawley rats were randomly divided into a control group ( n=35), an intervention group ( n=35) and a sham group ( n=9). Allen′s method was used to establish a contusion model of SCI in the rats of the control and intervention groups, while the sham group′s spinal tissues were exposed but not stricken. Beginning twenty-four hours after SCI modeling, the intervention group was given 7min of USW therapy daily, five days a week till the day of sacrifice for sampling the target area of spinal cord for tests. Then, motion function was evaluated using Basso, Beattie and Bresnahan (BBB) scoring. One, three and seven days after the SCI modeling, immunofluorescence and western blotting were employed to observe any changes in inflammatory factors and the MAPK pathway in the lesioned area. Results:Fourteen days after the modeling the average BBB score of the intervention group was significantly higher than the control group′s average. Moreover, 7 days after the modeling the average content of the domains containing protein 3 (NLRP3), interleukin-6 (IL-6), IL-6 receptor and tumor necrosis factor-α (TNF-α) in the target area of the spinal cord of sham group showed significantly lower levels than in the other 2 groups. And the levels in the intervention group were significantly lower than in the control group. Seven days after the modeling the number of cells positive for zinc finger protein 36 (TTP) in the lesioned area of the intervention group was significantly greater than among the control group. At the same time the levels of MAPK-activated protein kinase 2 (MK2), phosphorylated-mitogen-activated protein kinase-activated version (p-MK2) and TTP in the control and intervention groups were significantly higher than in the sham group. And there were significant differences between the intervention group and control group in the levels of MK2, p-MK2 and TTP.Conclusion:Ultrashortwave therapy can inhibit inflammation by regulating the MAPK inflammatory pathway, promoting the recovery of motion functions, at least in rats.
