Detection of Malignant Cells in Pleural Fluid or Ascites by CD44v8-10/CD44v10 competitive RT-PCR.
- Author:
Myung Ju AHN
1
;
Yun Hee NOH
;
Ho Ju YOON
;
Suck Cheol YANG
;
Jang Won SOHN
;
Jung Hae CHOI
;
Young Yeul LEE
;
Il Young CHOI
;
In Soon KIM
;
Yong Sung LEE
;
Chan Kum PARK
Author Information
1. Department of Internal Medicine, College of Medicine Hanyang University.
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Malignant effusion;
Malignant ascites;
CD44v8-10/CD44v10 competitive reverse transcription- polymerase chain reaction
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Antigens, CD44/analysis*;
Ascites/pathology*;
Ascites/immunology*;
Base Sequence;
Carcinoma, Non-Small-Cell Lung/pathology;
Carcinoma, Non-Small-Cell Lung/immunology;
Comparative Study;
Female;
Gastrointestinal Neoplasms/pathology;
Gastrointestinal Neoplasms/immunology;
Human;
Lung Neoplasms/pathology;
Lung Neoplasms/chemistry;
Male;
Middle Age;
Molecular Sequence Data;
Pleural Effusion, Malignant/pathology*;
Pleural Effusion, Malignant/chemistry*;
Reverse Transcriptase Polymerase Chain Reaction*;
Sensitivity and Specificity;
Support, Non-U.S. Gov't
- From:The Korean Journal of Internal Medicine
2001;16(1):30-35
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: CD44 is a cell surface adhesion molecule which has been implicated in various biologic functions as lymphocyte homing and activation, cellular migration and extracellular matrix adhesion. Over-expression of CD44v8- 10 has been found in several cancers and is considered to be associated with tumor progression and metastasis. Recently, a novel molecular method, CD44v8- 10/CD44v10 competitive reverse transcription-polymerase chain reaction(RT-PCR) has been developed for detecting cancer cells over-expressing CD44v8-10. METHODS: We analyzed from benign and malignant pleural effusion and ascites by CD44 competitive RT-PCR and compared to the conventional cytology. RESULTS: The CD44 competitive RT-PCR analysis showed that all the 24 samples associated with benign disease presented a predominant expression of the CD44v10 transcript (v8-10/v10 ratio: 0.126-0.948), whereas 6 of 7 malignant pleural samples associated with cytology positive cancer expressed the CD44v8-10 transcript (v8-10/v10 ratio > 1.00). CONCLUSION: These results indicate that CD44 competitive RT-PCR assay is a useful and adjunct to cytological examination in cancer diagnosis, especially in detecting exfoliated cancer cells in pleural effusion.
