Expression of TGF-alpha, beta1, beta2 in Pterygial Mast cell.
- Author:
Tae Hyung KIM
1
;
Seong Wook SEO
;
Jun Kyung SONG
Author Information
1. Department of Ophthalmology, College of Medicine, Gyeongsang National University, Korea. stramast@gshp.gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
TGF-alpha;
beta1;
beta2;
Mast cell;
Pterygium
- MeSH:
Conjunctiva;
Humans;
Immunohistochemistry;
Mast Cells*;
Paraffin;
Pterygium;
Tolonium Chloride;
Transforming Growth Factor alpha*;
Transforming Growth Factor beta1
- From:Journal of the Korean Ophthalmological Society
2002;43(7):1261-1268
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate whether TGF-alpha, beta1, beta2 are expressed in pterygial mast cell and the difference between each growth factor exists. METHODS: We prepared 10 pieces each of excised normal conjunctiva, primary pterygium, recurrent pterygium from human eyes and then fixed those in 4% paraformaldehyde solution. The tissues were embedded in paraffin and slide samples taken from those. After that, we observed mast cells stained by immunohistochemistry against each of TGF-alpha,beta1 ,beta2 and compared the staining features between them, and we also confirmed metachromatic staining of the mast cell with toluidine blue. RESULTS: As compared with normal conjunctiva, in primary and recurrent pterygia, those counts of mast cells expressing TGF-alpha, beta1, beta2 were increased respectively with statistical significance and especially, those counts for TGF-beta1 in primary pterygium were 9 times as high as in normal conjunctiva. CONCLUSIONS: Pterygial mast cells are considered to play an important role in fibrovascular proliferation of pterygium by synthesizing and secretion of TGF-alpha, beta1, beta2 . Especially, TGF-beta1 is thought to play a more important role than TGF-alphaandbeta2 in primary pterygium.
