Influence of donor and pancreatic characteristics on the success rate of islet isolation
10.3760/cma.j.cn311282-20220922-00549
- VernacularTitle:供体因素和胰腺特点对胰岛制备成功率的影响
- Author:
Jiaqi ZOU
1
;
Xingheng CAI
;
Peng SUN
;
Xuejie DING
;
Shusen WANG
Author Information
1. 天津市第一中心医院器官移植中心，天津 300192
- Keywords:
Donor characteristics;
Islet isolation;
Islet transplantation;
Success rate
- From:
Chinese Journal of Endocrinology and Metabolism
2023;39(5):421-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the selection criteria of the donor for islet transplantation of Chinese people by analyzing the correlation between pancreas characteristics and success rate of islets isolation.Methods:Data from 113 cases of human islet isolation were collected. According to the result of islet isolation, the donors were divided into two groups, the success group(IEQ≥250 000, purification≥30%, and viability≥80%), and the failure group(IEQ<250 000, or purification<30%, or viability<80%). The modified Ricordi method was used to digest pancreas tissue, and the continuous density gradient method was performed to purify islets. The islets were identified by staining with the Dithizone(DTZ), the islets were analyzed for cell viability and purity.Results:The donor age in success group was significantly younger than failure group in the range of age eligible for this study( t=2.479, P=0.015). Pearson correlation showed that donor age was positively corelated with islet yield( r=-0.214, P=0.047). There was more fat on the pancreas surface in the successful islet isolation group( z=-2.007, P=0.045). The digestibility( t=2.133, P=0.035) and recovery rate( t=5.912, P=0.001) were elevated in success group. Conclusion:The pancreases from younger donors could obtain the higher-yielding islet, the pancreas with more surface fat or with higher weight was associated with islet isolation success in the scope covered by the inclusion criteria of this study.
