Application of single cell RNA sequencing in the treatment of diabetes
10.3760/cma.j.cn311282-20220301-00120
- VernacularTitle:单细胞RNA测序在糖尿病治疗中的应用
- Author:
Caiping ZHOU
1
;
Hong LI
;
Xihua LIN
Author Information
1. 浙江大学医学院附属邵逸夫医院内分泌科，杭州 310016
- Keywords:
Single cell RNA sequencing;
Diabetes mellitus;
Islet β cells;
Diabetic complications
- From:
Chinese Journal of Endocrinology and Metabolism
2023;39(2):165-171
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell RNA sequencing (scRNA-seq) is used for transcriptome profiling at the individual cell level, which is capable of screening in differentially gene expression that results from genetic mutation. Islet-based developmental atlas and heterogeneity characterization are currently the main applications of scRNA-seq in diabetes. scRNA-seq also can be used to mark and purify the functional β cells from resident adult stem cells in the pancreatic islets, which is expected to improve the outcome of islet β cells transplantation in type 1 diabetic patients. In addition, the technique can aid in learning diabetic β cell dedifferentiation and immunomodulatory functions. Although the study of scRNA-seq in diabetic retinopathy, nephropathy, atherosclerosis, and peripheral neuropathy is still at a nascent stage, scRNA-seq has great potential in a wide range of biomedical and clinical applications.
