Role of orphan nuclear receptor Nur77 in tunicamycin-induced injury to hippocampal neurons of mice: relationship with endoplasmic reticulum stress
10.3760/cma.j.cn131073.20221216.00406
- VernacularTitle:孤儿核受体Nur77在衣霉素诱导小鼠海马神经元损伤中的作用：与内质网应激的关系
- Author:
Xiaoye WANG
1
;
Tianya LIU
;
Siqi ZHOU
;
Jingjing DANG
;
Zhiping WANG
Author Information
1. 徐州医科大学 江苏省麻醉学重点实验室，徐州 221004
- Keywords:
Nuclear receptor subfamily 4, group a, member 1;
Tunicamycin;
Hippocampus;
Neurons;
Endoplasmic reticulum stress
- From:
Chinese Journal of Anesthesiology
2023;43(4):418-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of orphan nuclear receptor Nur77 in tunicamycin(TM)-induced injury to hippocampal neurons and the relationship with endoplasmic reticulum stress in mice.Methods:Mouse HT22 cells were divided into 4 groups ( n=10 each) using a random number table method: control group (C group), Nur77 specific agonist Csn-B group (Csn-B group), endoplasmic reticulum stress inducer TM group (TM group), and TM+ Csn-B group. Cells in C group were cultured for 24 h under normal condition. In Csn-B group, Csn-B at a final concentration of 10 μg/ml was added to the culture medium, and the cells were incubated for 24 h. In TM group, TM at a final concentration of 200 ng/ml was added to the culture medium and the cells were incubated for 24 h to induce cell endoplasmic reticulum stress injury. Cells in TM+ Csn-B group were pretreated with Csn-B at a final concentration of 10 μg/ml for 15 min, then TM at a final concentration of 200 ng/ml was added, and the cells were co-incubated for 24 h. The cell viability was examined by CCK-8 assay kit after treatment in each group. The expression of endoplasmic reticulum stress-related protein CCAAT/enhancer-binding protein homologous protein (CHOP), glucose regulated protein 78 (GRP78)and apoptosis-associated protein Bcl-2, Bax, caspase-3 and cleaved-caspase-3 was detected by Western blot. Results:Compared with C group, the cell viability was significantly decreased, and the expression of CHOP, GRP78, Bax and cleaved-caspase-3 was up-regulated, and the expression of Bcl-2 and caspase-3 was down-regulated in TM group ( P<0.05 or 0.01). Compared with TM group, the cell viability was significantly increased, the expression of CHOP, GRP78, Bax and cleaved-caspase-3 was down-regulated, and the expression of Bcl-2 and caspase-3 was up-regulated in TM+ Csn-B group ( P<0.05 or 0.01). Conclusions:Orphan nuclear receptor Nur77 is involved in TM-induced injury to hippocampal neurons, which is related to activation of the endoplasmic reticulum stress in mice.
