Role of NALCN in hippocampal dentate gyrus in social behavior of mice
10.3760/cma.j.cn131073.20220828.00312
- VernacularTitle:海马齿状回NALCN在小鼠社交行为中的作用
- Author:
Lijuan WANG
1
;
Xiaoyuan GENG
;
Bo YANG
;
Yu ZHANG
;
Ning DING
;
Na SUN
;
Tao WANG
;
Junbo ZHAO
;
Lihua JIANG
Author Information
1. 郑州大学第三附属医院麻醉科，郑州 450000
- Keywords:
Dentate gyrus;
Sodium channels;
Social communication disorder
- From:
Chinese Journal of Anesthesiology
2023;43(3):313-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of the sodium leak channel (NALCN) in the hippocampal dentate gyrus (DG) in the social behavior of mice.Methods:Thirty-nine male wild-type C57BL/6 mice, aged 6-8 weeks, weighing 18-22 g, were used in this study. Three mice were sacrificed to verify the expression and co-expression of NALCN with neuronal nuclear antigen (NeuN) in the hippocampal DG using the immunofluorescent staining. The remaining 36 mice were divided into 2 groups ( n=18 each) by the random number table method: control group (group C) and NALCN gene knockdown group (group KO). NALCN-shRNA virus was injected in group KO, and scrambled-shRNA virus was injected in group C. The three box social test and open field test were performed at 3 weeks after the virus injection. Mice were sacrificed under anesthesia after the behavioral test, hippocampal tissues were collected, and the injection location of the virus was verified with a fluorescence microscope, and the NALCN protein and mRNA expression in the hippocampal DG was detected by Western blot and real-time polymerase chain reaction, respectively. Results:NALCN and NeuN co-expressed a lot on the same neuron in the hippocampal DG of mice, indicating that NALCN was widely expressed on the neurons in the hippocampal DG. Compared with group C, the expression of NALCN and mRNA in the hippocampal DG was significantly down-regulated, and the social novelty preference disappeared ( P<0.05), and no significant change was found in the social ability and each parameter in the open field test in group KO ( P>0.05). Conclusions:NALCN in the hippocampal DG is involved in the regulation of social memory in mice, and the down-regulated expression of NALCN can lead to the loss of social novelty preference in mice.
