Identification of Anthocyanin from The Extract of Soybean Seedcoat.
- Author:
Sun Mi PARK
1
;
Jina KIM
;
Tran Huu DUNG
;
Le Thanh DO
;
Do Thi Anh THU
;
Mi Kyung SUNG
;
Jong Sang KIM
;
Hoon YOO
Author Information
1. Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chosun University, Gwangju 501-759, Korea. hoon_yoo@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
anthocyanin / anthocyanidin;
soybean seedcoat;
TLC;
HPLC;
CZE
- MeSH:
Anthocyanins;
Chromatography, High Pressure Liquid;
Chromatography, Thin Layer;
Electrophoresis, Capillary;
Flowers;
Fruit;
Hydrogen-Ion Concentration;
Hydrolysis;
Retention (Psychology);
Soybeans
- From:International Journal of Oral Biology
2011;36(2):59-64
- CountryRepublic of Korea
- Language:English
-
Abstract:
Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis. The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin / anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside), with the relative order of anthocyanidin in cyanidin > delphinidin > petunidin > pelargonidin.
