Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046.
- Author:
Min Jung KIM
1
;
Jeong Bum MIN
;
Sun A LIM
;
Joong Ki KOOK
Author Information
1. Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seosuk-Dong, Dong-Ku, Gwangju 501-759, Korea. jkkook@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Detection;
Prevotella intermedia ATCC 49046;
strain-specific PCR primers
- MeSH:
Base Sequence;
DNA;
Limit of Detection;
Polymerase Chain Reaction;
Prevotella;
Prevotella intermedia;
Sprains and Strains
- From:International Journal of Oral Biology
2011;36(2):79-82
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.
