Automatic radiolabeling of the norepinephrine transporter targeted tracer 18F-mFBG and evaluation of 18F-mFBG PET/CT imaging in pheochromocytoma
10.3760/cma.j.cn321828-20230113-00008
- VernacularTitle:去甲肾上腺素转运蛋白显像剂 18F－mFBG的自动化合成及其用于嗜铬细胞瘤PET/CT显像的效果评估
- Author:
Weijian YE
1
;
Jie MA
;
Chenchen DONG
;
Junqi HU
;
Guocong LI
;
Junjie WEI
;
Ke ZHONG
;
Hao XU
;
Lu WANG
Author Information
1. 暨南大学附属第一医院核医学科，广州 510630
- Keywords:
Fluorobenzenes;
Norepinephrine plasma membrane transport proteins;
Isotope labeling;
Fluorine radioisotopes;
Pheochromocytoma;
Positron-emission tomography
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2023;43(9):543-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To fulfill the automatic radiolabeling of the norepinephrine transporter (NET) trancer 18F-meta-fluorobenzylguanidine (mFBG), and explore the 18F-mFBG PET/CT imaging effect of pheochromocytoma. Methods:On the basis of the chemical structure of mFBG, a spirocyclic iodonium ylide was used as the precursor to undergo a 3-step reaction sequence (radiofluorination, deprotection and neutralization) on AllinOne synthesis module. Purification by high performance liquid chromatography and formulation were conducted to generate 18F-mFBG. The corresponding quality control tests of 18F-mFBG product was performed. Afterwards, a postoperative patient with pheochromocytoma underwent 18F-mFBG PET/CT imaging. Results:The radiosynthesis was accomplished within 70 min, and 18F-mFBG was obtained in (17.8±2.4)% non-decay-corrected radiochemical yield ( n=5), with radiochemical purity >97% and molar activity >59.2 GBq/μmol. Sterility test, bacterial endotoxins test, abnormal toxicity test and the acetonitrile residue all met the requirements of Pharmacopoeia of the People′ s Republic of China (2020 Volume Ⅳ). The 18F-mFBG PET/CT imaging disclosed high uptake in pheochromocytoma and clear localization of lesions. Conclusions:The automatic radiolabeling of the NET targeted tracer 18F-mFBG is successfully realized by commercially available synthesis module, and the production quality meets all requirements for clinical translation. 18F-mFBG has a potential to image neuroendocrine lesions in clinical setting.
