Effect of hyperthermia on biological behavior of squamous cell carcinoma of the tongue by regulating ferroptosis pathway
10.3760/cma.j.cn113030-20220620-00222
- VernacularTitle:热疗调控铁死亡通路对舌鳞癌细胞生物学行为影响的研究
- Author:
Pei SHEN
1
;
Yuli HAO
;
Xuexiao ZHOU
;
Yuan CONG
;
Shengzhi WANG
;
Yun SHAO
;
Ting XU
;
Shouyi LI
Author Information
1. 青岛大学口腔医学院,青岛 266003
- Keywords:
Hyperthermia;
Squamous cell carcinoma of the tongue;
Ferroptosis;
Ferrostain-1
- From:
Chinese Journal of Radiation Oncology
2023;32(3):260-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulation and possible mechanism of hyperthermia (HT) on the ferroptosis of squamous cell carcinoma of the tongue cell line CAL-27.Methods:Half maximal inhibitory concentration (IC 50) of Fer-1, an inhibitor of ferroptosis, was detected by CCK-8 assay and used for subsequent experiments. CAL-27 cells were divided into the HT, control, Fer-1 and HT+ Fer-1 groups according to experimental design. Reactive oxygen species (ROS) levels and iron ion concentration were determined by corresponding detection kits. The p53 and TfR1 mRNA levels were detected by real-time reverse transcription PCR. Cell migration was detected by cell scratch test and cell apoptosis was detected by flow cytometry. Results:HT significantly up-regulated the ROS levels ( P<0.01) and iron ion concentration ( P<0.001), and significantly increased the expression levels of p53 and TfR1 mRNA (both P<0.01). The cell migration ability was decreased ( P<0.001), whereas cell apoptosis rate was increased by HT ( P<0.01). In the HT+Fer-1 group, the ROS levels ( P<0.001), iron ion concentration ( P<0.001), expression levels of p53 and TfR1 mRNA (both P<0.01) were significantly down-regulated, the cell migration ability was recovered ( P<0.01), and cell apoptosis rate was decreased ( P<0.01) compared with those in the HT group, respectively. Conclusions:HT may induce the ferroptosis of CAL-27 cell line, inhibit cell migration ability and promote cell apoptosis by activating the p53/TfR1 pathway.
