Effect of radiofrequency radiation from 5G mobile phone on blood-brain barrier in mice
10.3760/cma.j.cn112271-20221025-00418
- VernacularTitle:5G手机通信频段射频辐射对小鼠血脑屏障的影响
- Author:
Guiqiang ZHOU
1
;
Yizhe XUE
;
Zhaowen ZHANG
;
Tongzhou QIN
;
Ling GUO
;
Panpan LAI
;
Peng GAO
;
Xing WANG
;
Zhifei HUANG
;
Yuhang ZANG
;
Yuntao JING
;
Guirong DING
Author Information
1. 潍坊医学院公共卫生学院，潍坊 261053
- Keywords:
5G mobile phone;
Radio frequency radiation;
Mice;
Blood brain barrier
- From:
Chinese Journal of Radiological Medicine and Protection
2023;43(3):176-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of radiofrequency radiation (RF) from 5G mobile phone communication frequency bands (3.5 GHz and 4.9 GHz) on the permeability of the blood-brain barrier (BBB) in mice.Methods:A total of 24 healthy adult male C57BL/6 mice (6-8 weeks old) were randomly divided into Sham, 3.5 GHz RF and 4.9 GHz RF groups, and 8 mice in each group. Mice in the RF groups were systemically exposed to 5G cell phone radiation for consecutive 35 d(1 h/d) with 50 W/m 2 power density. The BBB permeability of mice was detected by Evans Blue (EB) fluorescence experiment. The expression levels of the BBB tight junction-related proteins (ZO-1, occludin and claudin-11) and the gap junction-related protein Connexin 43 were determined by Western blot. Results:The number of spots, fluorescence intensity and comprehensive score of EB were significantly increased in 3.5 GHz RF group and 4.9 GHz RF group compared with the Sham group ( t=12.98, 17.82, P<0.001). Compared with the Sham group, the content of S100B in mouse serum was significantly increased in 3.5 GHz RF group and 4.9 GHz RF group ( t=19.34, 14.68, P<0.001). The BBB permeability was increased in the RF group. The expression level of occludin protein was significantly reduced in the 3.5 GHz RF group ( t=-3.13, P<0.05), and this decrease was much profound in the 4.9 GHz RF group ( t=-6.55, P<0.01). But the protein levels of ZO-1, Claudin-11 and Connexin 43 in the cerebral cortex of the RF groups had no significantly difference in comparison with the Sham group( P>0.05). Conclusions:The continuous exposure of mobile phone RF at 3.5 GHz or 4.9 GHz for 35 d (1 h/d) induces an increase of BBB permeability in the mouse cerebral cortex, perhaps by reducing the expression of occludin protein.
