The radiosensitization effect of STING agonist on cutaneous melanoma cells
10.3760/cma.j.cn112271-20220826-00350
- VernacularTitle:干扰素基因激活因子激动剂对人皮肤黑色素瘤细胞的放射增敏作用研究
- Author:
Shaokai TANG
1
;
Jianhui CHEN
;
Fenghao GENG
;
Tingyi YANG
;
Xiaoqian LI
;
Xiaobo DU
;
Shuyu ZHANG
Author Information
1. 四川大学华西基础医学与法医学院放射医学研究室，成都 610041
- Keywords:
Cutaneous melanoma;
Stimulator of interferon gene;
Radiosensitivity
- From:
Chinese Journal of Radiological Medicine and Protection
2023;43(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the radiosensitizing effect and underlying mechanism of STING agonist (c-di-AMP) on cutaneous melanoma cells.Methods:Human cutaneous melanoma cells (A375) were divided into four groups: the control group, 10 μmol/L c-di-AMP group, X-ray irradiation group and X-ray irradiation combined with c-di-AMP group. The radiosensitizing effect of c-di-AMP on A375 cells was detected by CCK-8-based viability assay, lactate dehydrogenase (LDH) release assay, flow cytometry-based apoptosis assay, and colony formation assay. Western blot analysis was used to determine the expressions of cell death-related proteins.Results:In combination with 10 Gy X-ray irradiation, 10 μmol/L c-di-AMP showed significant radiosensitization effect in A375 cells, which was evidenced by decreased cell activity ( t=5.11, P<0.05), increased cytotoxicity ( t=10.15, P<0.05) and cell apoptosis ( t=4.41, P<0.05) and reduced clone viability( t=6.30, 3.55, 5.45, 3.55, P<0.05). The calculated radiosensitization ratio of c-di-AMP to A375 cells was 1.88. Moreover, 10 μmol/L c-di-AMP further increased the expressions of cell death-related proteins induced by radiation in A375 cells. Conclusions:The STING agonist c-di-AMP can be used as a radiosensitizer for cutaneous melanoma, which may provide a novel strategy for radiotherapy.
