Ionizing radiation promotes epithelial-mesenchymal transition of cervical cancer cell line Siha through increasing the secretion of exosomes
10.3760/cma.j.cn112271-20220908-00366
- VernacularTitle:电离辐射通过诱导外泌体的分泌促进宫颈癌Siha细胞上皮间质转化
- Author:
Lingli LIAO
1
;
Fan YANG
;
Yuwei MA
;
Luyao WANG
;
Zhen QU
;
Xiaojing WANG
;
Hao JIANG
;
Yongchun ZHOU
Author Information
1. 蚌埠医学院第一附属医院肿瘤放疗科 233000
- Keywords:
Cervical cancer;
Ionizing radiation;
Epithelial-mesenchymal transition;
Exosomes
- From:
Chinese Journal of Radiological Medicine and Protection
2022;42(12):922-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the occurrence of epithelial-mesenchymal transition (EMT) in cervical cancer cell line Siha irradiated by X-rays with clinical conventional fraction radiotherapy model and investigate the role of exosomes in this process.Methods:Siha cells were irradiated by 6 MV-X rays with 50 Gy in 25 fractions. EMT was evaluated by cell morphology, EMT biomarkers and cell migration and invasion ability. Exosomes released from cells were detected by transmission electron microscopy and nanoparticle tracking analysis (NTA), and its function in EMT was explored by using an exosome inhibitor GW4869 (10 μmol/L).Results:After irradiation, EMT phenomenon was induced in the survived Siha cells, including the incidence of mesenchymal phenotype, upregulation of epithelial marker E-cadherin ( t=9.66, P<0.05), downregulation of mesenchymal marker N-cadherin ( t=41.61, P<0.05), and increase of cell migration and invasion abilities ( t=6.11, 13.22; P<0.05). Meanwhile, the secretion of exosomes was also increased after irradiation ( t=7.51, P<0.05). When the cells were pre-treated with GW4869, radiation-induced exosome secretion was reduced ( t=7.28, P<0.05), so that radiation-induced EMT was reversed. Conclusions:Ionizing radiation with clinical conventional fraction radiotherapy model promotes EMT of cervical cancer cells through increasing the secretion of exosomes.
