Histopathological and immunohistochemical changes of gastric fundus and duodenum after bariatric embolization in the New Zealand rabbits
10.3760/cma.j.cn112149-20220321-00261
- VernacularTitle:新西兰白兔胃左及胃十二指肠动脉栓塞减肥术后组织病理及免疫组织化学变化
- Author:
Jinxin FU
1
;
Feng DUAN
;
Jinlong ZHANG
;
Bing YUAN
;
Heng ZHANG
;
Jieyu YAN
;
Yang GUAN
;
Yan WANG
;
Kai YUAN
;
Maoqiang WANG
Author Information
1. 解放军总医院第五医学中心介入放射科，北京 100039
- Keywords:
Radiology, interventional;
Left gastric artery embolization;
Histopathology;
Immunohistochemistry;
Ghrelin
- From:
Chinese Journal of Radiology
2023;57(3):306-310
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the histopathological and immunohistochemical changes of gastric fundus and duodenum after bariatric embolization of left gastric artery and gastroduodenal artery in obesity New Zealand rabbit models.Methods:Twenty obesity New Zealand rabbit models were successfully established and divided into two groups using stratified randomization. Left gastric artery and gastroduodenal artery were embolized with gelatin sponge (350-560 μm) in experimental group, left gastric artery and gastroduodenal artery were perfused with normal saline in control group. All animals were sacrificed for pathological, immunohistochemical examination and Western Blot analysis 4 weeks post embolization, the density of ghrelin producing cells and the gray ratio of ghrelin protein band were measured and compared by the independent sample t test. Results:Macropathological examination showed ulceration in the anterior wall of the gastric body in one rabbit, histopathological examination showed mucosa ulceration in the gastric body in 3 rabbits in experimental group. Immunohistochemical examination showed that the ghrelin producing cells of gastric fundus and duodenum in the experimental group were significantly less than those in the control group (10.0±5.1 vs.27.7±3.4, t=12.35, P<0.05;5.6±2.6 vs. 12.3±2.1, t=4.73, P<0.05). Western Blot analysis showed that the gray ratio of ghrelin bands of gastric fundus and duodenum in the experimental group were significantly lower than that in the control group (0.65±0.05 vs.1.12±0.09, t=9.62, P<0.05;0.55±0.03 vs. 0.94±0.08, t=7.98, P<0.05). Conclusions:Immunohistochemical and Western Blot analysis showed that the ghrelin-producing cells of gastric fundus and duodenum in the experimental group were significantly less than those in the control group after bariatric embolization, histopathologic analysis indicated that bariatric embolization was a safe technique.
