Antifungal Susceptibility Testing in Three-dimensional Keratinocyte Culture Model.
- Author:
Jeong Aee KIM
1
;
Jong Hee LEE
;
Dong Youn LEE
;
Sang Eun MOON
;
Kwang Hyun CHO
Author Information
1. Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea. jakim@snu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Antifungal susceptibility;
Dermatophyte;
Keratinocyte culture
- MeSH:
Antifungal Agents;
Arthrodermataceae;
Biological Availability;
Endpoint Determination;
Fluconazole;
Itraconazole;
Keratinocytes*;
Skin;
Trichophyton
- From:Korean Journal of Dermatology
2002;40(4):341-347
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Standardization of antifungal susceptibility testing for dermatophytes is important and several variables, such as inoculum size, length and temperature of incubation, media, and end point determination has recently been established. However, a more improved test model which can evaluate bioavailability and has clinical relevance is still needed. OBJECTIVE: We performed antifungal susceptibility testing by using three-dimensional keratinoctyte culture model, living skin equivalent (LSE), as an in vitro model. METHODS: LSE was prepared and various concentrations of antifungals were added to media. Microconidia of Trichophyton mentagrophytes was inoculated onto LSE and incubated for 6 days at 35 degrees C. RESULTS: Inhibition of fungal proliferation and invasion were observed at 0.1microgram/ml of terbinafine, 0.01 microgram/ml of itraconazole solution, 0.1 microgramg/ml of itraconazole powder, and 10 microgram/ml of fluconazole, respectively. CONCLUSION: Our culture model is similar to in vivo situation and the results were relatively well accordant to those of other previous studies. Our LSE model is considered as a promising in vitro system for evaluating the activity and safety of antifungal agents. However, further study using more various species of dermatophytes and more strains is still needed.
