The effect of electroacupuncture stimulation at acupoint Zusanli on the model of Staphylococcus aureus osteomyelitis
10.3760/cma.j.cn115530-20230227-00101
- VernacularTitle:电针刺激足三里在金黄葡萄球菌小鼠骨髓炎模型中的作用研究
- Author:
Yuchi YAN
1
;
Zixian LIU
;
Bin YU
Author Information
1. 南方医科大学南方医院骨科-创伤骨科，广东省骨与软骨再生医学重点实验室 广州，510515
- Keywords:
Osteomyelitis;
Mice;
Mesenchymal stem cell;
Acupoint Zusanli (ST36);
Staphylococcus aureus;
Electroacupuncture stimulation
- From:
Chinese Journal of Orthopaedic Trauma
2023;25(5):440-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether the electroacupunture stimulation (ES) at acupoint Zusanli (ST36) can inhibit the bone loss caused by Staphylococcus aureus (SA) infection and its mechanism in a model of SA osteomyelitis.Methods:Twelve male C57 BL/6 mice aged 10 to 12 weeks were randomly divided into 2 even groups ( n=6) for SA infection + ES or SA infection only. After ES at ST36 was conducted for 4 weeks in the model of SA osteomyelitis, samples were harvested from the femora and tibiae. Micro-CT reconstruction was performed to detect trabecular bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), connectivity density (Conn.Dn) to analyze changes in bone mass. Leptin receptor (LEPR) staining was performed to detect osteoblasts. Tartrate resistant acid phosphatase (TRAP) staining was used to detect the changes in osteoclasts. The changes in plasma inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). Results:Micro-CT results showed that the BV/TV, Tb.N, Tb.Th, and Conn.Dn in the cancellous bone in the target areas in the SA + ES group were all higher than those in the SA group, LEPR immunofluorescence results indicated that the number of osteogenic precursor cells in the ES group was larger than that in the SA group, and serum ELISA indicated a decrease in inflammatory factors in the blood in the SA+ES group compared with the SA group. There were significant differences in the comparisons above ( P<0.05). There was no significant difference in the number of osteoclasts on the surface of trabecular bone between the 2 groups in TRAP staining. Conclusion:ES may slow down infectious bone destruction by inhibiting the inflammatory response induced by SA infection and by inducing aggregation and differentiation of mesenchymal stem cells into trabecular bone.
