Effects of Alpha-2-macroglobulin-rich serum on knee post-traumatic osteoarthritis in rats
10.3760/cma.j.cn115530-20220504-00240
- VernacularTitle:富含α-2巨球蛋白的血清治疗大鼠膝关节创伤性骨关节炎的效果评价
- Author:
Xiaohu WANG
1
;
Xiaochun WEI
;
Pengcui LI
;
Lu LI
;
Ruipeng ZHAO
;
Zhiqing DUAN
Author Information
1. 山西医科大学第二医院骨科，太原 030001
- Keywords:
Alpha-macroglobulins;
Serum;
Osteoarthritis, knee;
Wounds and injuries;
Treatment outcome;
Rats
- From:
Chinese Journal of Orthopaedic Trauma
2022;24(12):1083-1087
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of Alpha-2-macroglobulin-rich serum (A2MRS) on knee post-traumatic osteoarthritis (PTOA).Methods:The knee PTOA models were constructed by transection of the anterior cruciate ligament (ACL) in 80 SD male rats, aged 2 months and weighing from 250 to 300 g, which were randomized into 4 groups ( n=20): a high dose group (A2MRS containing 20 μg/μL A2M administered), a low dose group (A2MRS containing 10 μg/μL A2M administered), a positive control group (normal saline administered), and a blank control group (the knee joint cut pseudooperatively and normal saline administered). HE, toluidine blue staining, safranine O staining, modified Mankin scoring and Osteoarthritis Research Society International (OARSI) scoring were conducted to evaluate and compare the therapeutic effects of A2MRS on the knee PTOA among the 4 groups. Results:The rat cartilage was thinner with patchy and cracked surface, and the chondrocytes were reduced and distributed unevenly in the positive control group, compared with the blank control group. The modified Mankin score (3.89±0.93) and OARSI score (10.05±0.72) in the positive control group were significantly higher than those in the blank control group (0.67±0.07 and 3.10±0.29) ( P<0.05). The rat cartilage was thicker with basically complete and crack-free surface, and the chondrocytes were increased and distributed more evenly in the high dose group and the low dose group, compared with the positive control group. The modified Mankin scores (1.33±0.50 and 1.56±0.53) and OARSI scores (6.30±0.64 and 4.75±0.66) in the high dose group and the low dose group were significantly lower than those in the positive control group ( P<0.05). However, there were no such differences between the high dose group and the low dose group ( P>0.05). Conclusion:A2MRS effectively delays the pathological process of knee PTOA.
