Etiological diagnosis value of metagenomic next generation sequencing in the patients with spinal infection
10.3760/cma.j.cn311365-20220513-00181
- VernacularTitle:宏基因组学二代测序对脊柱感染患者病原学诊断的价值
- Author:
Dongmei ZHANG
1
;
Weili ZHAN
;
Huiming JIN
;
Zhen PENG
;
Shuai DING
;
Dongqiang YANG
;
Yanhong KANG
;
Yuehua KANG
;
Jia SHANG
;
Erhui XIAO
Author Information
1. 河南大学人民医院 河南省人民医院感染科，郑州 450003
- Keywords:
Spinal infection;
Metagenomics next generation sequencing;
Pathogenic microorganism;
Diagnostic value
- From:
Chinese Journal of Infectious Diseases
2023;41(3):208-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the efficacy of metagenomic next generation sequencing (mNGS) in the etiological diagnosis of patients with spinal infection, so as to provide reference for timely diagnosis and treatment.Methods:A total of 40 patients with suspected spinal infection admitted to the Department of Infectious Diseases in Henan Provincial People′s Hospital from January 2020 to July 2022 were included. The results of tissue culture, histopathological examination and tissue mNGS detection were analyzed retrospectively. According to the clinical diagnose, the patients were divided into the spinal infection group (28 cases) and the non-spinal infection group (12 cases). The positive rate, sensitivity and specificity of mNGS and tissue culture in the pathogen detection of patients with spinal infection were compared. McNemar test was used for statistical analysis.Results:There were 23 males and 17 females in 40 patients. The positive rate of mNGS was higher than that of tissue culture (75.0%(30/40) vs 12.5%(5/40)), and the difference was statistically significant ( χ2=0.08, P<0.001). Based on clinical diagnostic criteria, the sensitivity of mNGS in the diagnosis of spinal infection was higher than that of tissue culture (82.1% vs 17.9%), with a statistically significant difference ( χ2=0.02, P<0.001), while the specificity compared to the tissue culture (33.3% vs 100.0%), the difference was not statistically significant ( P>0.05). Conclusions:mNGS has a high pathogen detection rate and sensitivity in the etiological diagnosis of patients with spinal infection, which could provide clinical guidance for the diagnosis and treatment of patients with spinal infection.
