Role of circTmeff-1 in morphine addiction memory of mice
10.3867/j.issn.1000-3002.2023.07.145
- Author:
Hailei YU
1
;
Boyang WEN
;
Yun LU
;
Bing XIE
;
Feng YU
;
Minglong ZHANG
;
Chunling MA
;
Bin CONG
;
Di WEN
;
Haitao BI
Author Information
1. College of Forensic Medicine,Hebei Medical University,Hebei Key Laboratory of Forensic Medicine,Collaborative Innovation Center of Forensic Medical Molecular Identifica-tion,Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology,Chinese Academy of Medi-cal Sciences,Shijiazhuang 050011,China
- Keywords:
circRNAs;
morphine;
conditional place pref-erence;
addiction memory;
nucleus accumbens
- From:
Chinese Journal of Pharmacology and Toxicology
2023;37(7):539-540
- CountryChina
- Language:Chinese
-
Abstract:
In addition to the essential pharmacologi-cal effects of opioids,situational cues associated with drug addiction memory are key triggers for drug seeking.CircRNAs,an emerging hotspot regulator in crown genet-ics-play an important role in central nervous system-relat-ed diseases.However,the internal mediating mechanism of circRNA in the field of drug reward and addiction mem-ory remains unknown.Here,we trained mice on a condi-tional place preference(CPP)model and collected nucle-us accumbens(NAc)tissues from day 1(T0)and day 8(T1)for high-throughput RNA sequencing.qRT-PCR revealed that circTmeff-1 was highly expressed in the NAc core but not in the NAc shell,suggesting that it plays a role in addiction memory formation.Meanwhile,the reverse regulation of circTmeff-1 by adeno-associated viruses could both inhibit the formation of addiction mem-ory in the NAc core or shell.Subsequently,the GO and KEGG analyses indicated 21 that circTmeff-1 might regu-late the addiction memory via the MAPK and AMPK path-ways.These findings suggest that circTmeff-1 in NAc plays a crucial role in morphine-dependent memory for-mation.
