The role of status-related neuronal ensembles in tem-poral lobe epilepsy
10.3867/j.issn.1000-3002.2023.07.003
- Author:
Nanxi LAI
1
;
Heming CHENG
;
Zhisheng LI
;
Zhong CHEN
;
Yi WANG
Author Information
1. Institute of Pharmacology&Toxicology,College of Phar-maceutical Sciences,Zhejiang University,Hangzhou 310058,China
- Keywords:
epilepsy;
neural ciruti;
piriform cortex;
activity responsive;
therapeutic target
- From:
Chinese Journal of Pharmacology and Toxicology
2023;37(7):481-482
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE Epileptic networks are char-acterized as two states,seizures or more prolonged inter-ictal periods.However,cellular mechanisms underlying the contribution of interictal periods to ictal events remain unclear.METHODS Here,we present the procedure for labeling seizure-activated and interictal-activated neuro-nal ensembles in mouse hippocampal kindling model using an enhanced-synaptic-activity-responsive element.This technique is combined with genetically encoded effectors to characterize and manipulate neuronal ensembles recruited by focal seizures(FS-Ens)and interictal periods(IP-Ens)in piriform cortex,a region that plays a key role in seizure generation.RESULTS Ca2+ activities and histo-logical evidence reveal a disjointed correlation between the two ensembles during FS dynamics.Optogenetic acti-vation of FS-Ens promotes further seizure development,while IP-Ens protects against it.Interestingly,both ensem-bles are functionally involved in generalized seizures(GS)due to circuit rearrangement.IP-Ens bidirectionally modulates FS but not GS by controlling coherence with hippocampus.CONCLUSION This study indicates that the interictal state may represent a seizure-preventing environment,and the interictal-activated ensemble may serve as a potential therapeutic target for epilepsy.
