Further Characterization of Activin A-induced IgA Response in Murine B Lymphocytes.
- Author:
Hwa Joung LEE
1
;
Pyeung Hyeun KIM
Author Information
- Publication Type:Original Article
- Keywords: activin A; TGF-beta; IgA; mesenteric lymph node; CFSE
- MeSH: Activins; Animals; B-Lymphocytes; Cell Division; Cell Survival; Fluoresceins; Immunoglobulin A; Lymph Nodes; Mice; Succinimides; Transforming Growth Factor beta; Transforming Growth Factor beta1
- From:Immune Network 2009;9(4):133-137
- CountryRepublic of Korea
- Language:English
- Abstract: We have recently shown that activin A, a member of TGF-beta superfamily, stimulates mouse B cells to express IgA isotype but other isotypes. In the present study, we further characterized effects of activin A on B cell growth and IgA expression. We found that activin A did not have effect on LPS-stimulated cell viability. In parallel, CFSE staining analysis revealed that activin A did not alter cell division. An increase of IgA secretion by activin A was completely abrogated by anti-activin A Ab but not by anti-TGFbeta1 Ab. In the same conditions, no other isotypes are significantly affected by each antibody treatment. Finally, activin A, as similar to TGF-beta1, increased IgA secretion by mesenteric lymph node cells. These results suggest that activin A can specifically stimulate IgA response, independent of TGF-beta in the gut.
