Confusion and countermeasures of the non-conditioned anxiety test in rodent laboratory animals
10.3760/cma.j.cn371468-20221107-00661
- VernacularTitle:啮齿类动物非条件性焦虑测试的困惑与对策
- Author:
Kun LIU
1
;
Ying XING
;
Zhiyong ZHENG
;
Jialing XU
;
Hao ZHANG
;
Minghui HU
;
Xiaohua HE
;
Kai CHEN
;
Zifa LI
;
Xiwen GENG
;
Sheng WEI
Author Information
1. 山东中医药大学中医药经典理论教育部重点实验室，山东中医药大学中医药与脑科学实验平台，济南 250355
- Keywords:
Non-conditioned anxiety;
Behavioral assessment;
Open field test;
Elevated plus maze;
Light-dark box
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2023;32(4):361-366
- CountryChina
- Language:Chinese
-
Abstract:
Anxiety is a major mood disorder, and the high morbidity, co-morbidity and disability of anxiety disorders seriously affect people's quality of life, so the importance and urgency of research on anxiety cannot be overstated. Animal models are the main carriers for studying the mechanism of disease occurrence and development, drug efficacy evaluation and drug development.Unconditioned anxiety model is a common anxiety model.Elevated plus maze test, open field test and light-dark box test are widely accepted paradigms for the detection of unconditioned anxiety.This kind of behavioral paradigm based on environmental exposure takes advantage of the conflict between curiosity and fear of the unfamiliar environment to simulate and detect the anxiety of animals.However, the validity of these behavioral paradigms for evaluating anxiety in animals is questionable.In this paper, we discuss the concept of anxiety, the definition of anxiety behavior in the behavioral test of unconditioned anxiety, and the factors to be considered in the test of unconditioned anxiety behavior.On this basis, new solutions were proposed to the contradictions and blind spots in order to improve the test paradigm of anxiety behavior and provide a more reliable animal model for the evaluation of anxiety.This paper presents a new approach to address the contradictions and blind spots of this paradigm.
