Protective effect of total aralosides of Aralia elata (Miq) Seem (TASAES) against diabetic cardiomyopathy in rats during the early stage, and possible mechanisms.
10.3858/emm.2009.41.8.059
- Author:
Shugang XI
1
;
Guihua ZHOU
;
Xuexin ZHANG
;
Wenjie ZHANG
;
Lu CAI
;
Chunyan ZHAO
Author Information
1. Department of Endocrinology, at the First Hospital, Jilin University, Changchun 130021, China.
- Publication Type:Original Article
- Keywords:
araloside;
calcium channels, L-type;
cardiomyopathies;
connective tissue growth factor;
diabetes mellitus;
heart;
hemodynamics;
myocardium
- MeSH:
Animals;
Aralia/*chemistry;
Calcium Channels, L-Type/physiology;
Cardiomyopathies/*drug therapy/etiology/physiopathology;
Connective Tissue Growth Factor/metabolism;
Diabetes Mellitus, Experimental/*complications;
Drugs, Chinese Herbal/*chemistry;
Heart/drug effects/physiopathology;
Hemodynamics;
Male;
Myocardium/pathology;
*Oleanolic Acid/analogs & derivatives/therapeutic use;
Patch-Clamp Techniques;
Potassium Channels/physiology;
Rats;
Rats, Wistar;
Saponins/*therapeutic use;
Treatment Outcome
- From:Experimental & Molecular Medicine
2009;41(8):538-547
- CountryRepublic of Korea
- Language:English
-
Abstract:
Total aralosides of Aralia elata (Miq) Seem (TASAES) from Chinese traditional herb Longya Aralia chinensis L was found to improve cardiac function. The present study was to determine the protective effects of TASAES on diabetic cardiomyopathy, and the possible mechanisms. Therefore, a single dose of streptozotocin was used to induce diabetes in Wister rats. Diabetic rats were immediately treated with low, medium and high doses of TASAES at 4.9, 9.8 mg/kg and 19.6 mg/kg body weight by gavage, respectively, for eight weeks. Cardiac function was evaluated by in situ hemodynamic measurements, and patch clamp for the L-type Ca2+ channel current (ICa2+-L) and transient outward K+ channel current (Ito). Histopathological changes were observed under light and electron microscope. The expression of pro-fibrotic factor, connective tissue growth factor (CTGF) was monitored using immunohistochemistry staining. Compared with diabetic group, medium and high doses, but not low dose, of TASAES showed a significant protection against diabetes-induced cardiac dysfunction, shown by increased absolute value of left ventricular systolic pressure (LVSP) and maximum rates of pressure development (+/-dp/dt(max)), and enhanced amplitude of ICa2+-L (P < 0.05). Histological staining indicated a significant inhibition of diabetes-caused pathological changes and up-regulation of CTGF expression (P < 0.05). The results suggest that TASAES prevents diabetes-induced cardiac dysfunction and pathological damage through up-regulating ICa2+-L in cardiac cells and decreasing CTGF expression.
