Genetic variation characteristics of HA gene of influenza A virus (H3N2) in Guizhou province from 2017 to 2019
10.3760/cma.j.cn341190-20220122-00065
- VernacularTitle:贵州省2017-2019年H3N2亚型流感病毒HA基因遗传特征分析
- Author:
Qinni ZHENG
1
;
Lijuan REN
;
Li ZHUANG
;
Chengzhong FU
;
Yonghu WAN
Author Information
1. 贵州省疾病预防控制中心实验中心病毒科，贵阳 550004
- Keywords:
Influenza A virus,H3N2 subtype;
RNA,viral;
Reverse transcriptase polymerase chain reaction;
Multilocus sequence typing;
Sequence homology,nucleic acid;
Her
- From:
Chinese Journal of Primary Medicine and Pharmacy
2023;30(5):692-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic variation characteristics of the HA gene of influenza A virus (H3N2) in Guizhou province from 2017 to 2019. Methods:Twenty strains of influenza A virus (H3N2) were randomly selected from 10 network laboratories in Guizhou province for RNA extraction. Reverse transcriptase-polymerase chain reaction and sequencing were performed. The products were analyzed using bioinformatics software.Results:The nucleotide homology of the HA gene of the 20 strains was 97.7%-100%, which was highly homologous to the vaccine strains A/Hong-Kong/4801/2014 recommended by WHO in 2017 and A/Singapore-INFIMH/16-0019/2016 recommended by WHO in 2018, but they were significantly different from the vaccine strain A/Kansas/14/2017 recommended by WHO in 2019. Genetic analysis showed that the 20 strains were divided into two branches, and the strains that were prevalent in 2019 were located in different branches, with marked genetic differences. Key site analysis showed mutations in antigenic determinants A, B, C, and E and mutations in the anterior and posterior walls of receptor binding sites. Key site analysis also showed that there was an increase in the number of glycosylation sites compared with the vaccine strains prevalent in the same year. Genetic distance, antigen sites, and glycosylation sites were slightly different between virus strains prevalent in 2017-2018 and virus strains prevalent in 2019. Conclusion:The HA gene of the influenza A virus subtype H3N2 in Guizhou province from 2017 to 2019 showed heterogeneity and gene mutation, especially in 2019. Therefore, close monitoring of the genetic evolution of the influenza A virus subtype H3N2 is necessary.
