Isolation and identification of plague phage from Suncus murinus in Yunnan Province and its epidemiological significance
10.3760/cma.j.cn231583-20220706-00245
- VernacularTitle:云南省大臭鼩鼠疫噬菌体的分离鉴定及其流行病学意义
- Author:
Xiaofei ZHAO
1
;
Liyuan SHI
;
Zongti SHAO
;
Chao SU
;
Shanshan DONG
;
Fengyi YANG
;
Peng WANG
;
Youhong ZHONG
Author Information
1. 云南省地方病防治所中心实验室 云南省自然疫源性疾病防控技术重点实验室，大理 671000
- Keywords:
Plague;
Suncus murinus;
Phage;
Epidemiology
- From:
Chinese Journal of Endemiology
2023;42(7):525-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the basic situation of Yunnan Province Suncus murinus carrying plague phage and to explore its epidemiological significance. Methods:From 2015 to 2018, a survey of plague host animals was carried out in 10 investigation sites in the historical plague foci, new plague foci (after 1982) and stubborn plague foci of domestric mouse in Yunnan Province. The plague phage was isolated and cultured from the intestinal specimens of Suncus murinus. The growth of plaque was observed by double-layer plate method, and the morphology and structure of plague phage were observed under electron microscope. At the same time, intestinal samples were taken to detect the structural gene caf1 of F1 antigen of Yersinia pestis. Results:In this study, a total of 157 Suncus murinus were captured and 16 strains of plague phage were isolated, with a total isolation rate of 10.19%. There was no difference in plague phage isolation rate between historical plague foci (10.00%, 1/10) and stubborn plague foci (16.22%, 12/74), new plague foci (4.11%, 3/73, χ 2 = 0.00, P = 0.965; Fisher test, P = 1.000). However, there was a difference in plague phage isolation rate between stubborn plague foci and new plague foci (χ 2 = 5.88, P = 0.015). There was no significant difference in the isolation rate of plague phage among different sex, growth period and habitat ( P > 0.05). The plaque morphology of the isolated plague phage was diverse, of which four strains were myotavirus phages; and all samples were negative for F1 antigen structural gene caf1. Conclusions:Suncus murinus is widely distributed in the domestic mouse plague foci in Yunnan Province, and the animals carry a certain number of plague phage. Regular surveillance of Suncus murinus and their plague phage has a certain guiding significance for the surveillance and early warning of plague in Yunnan Province.
