Phenotype and genotyping of Yersinia pestis in China
10.3760/cma.j.cn231583-20221215-00404
- VernacularTitle:中国鼠疫耶尔森菌表型及其基因分型研究
- Author:
Xuefei ZHANG
1
;
Jian HE
;
Wenyuan XIN
;
Juan JIN
;
Xiaoyan YANG
;
Sheng LI
;
Qi ZHANG
;
Youquan XIN
;
Qingwen ZHANG
;
Ruixia DAI
;
Baiqing WEI
Author Information
1. 青海省地方病预防控制所国家卫生健康委员会鼠疫防治研究重点实验室 青海省鼠疫防控及研究重点实验室，西宁 810021
- Keywords:
Yersinia pestis;
Plague natural foci;
Phenotypic characteristics;
Genotype
- From:
Chinese Journal of Endemiology
2023;42(7):517-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the phenotype and genotype distribution of Yersinia pestis ( Y. pestis) in different natural foci of plague in China, so as to provide scientific basis for plague prevention and control. Methods:A total of 2 184 strains of Y. pestis isolated from different time periods, regions, hosts and vectors in 11 plague natural foci of China since 1943 were selected for biochemical type identification, glycolysis test, virulence factor test [capsule antigen (F1), pesticin Ⅰ (Pst Ⅰ), virulence antigen factor (VWa), pigmentation factor (Pgm)], different region (DFR) typing and clustered regularly interspaced short palindromic repeats (CRISPR) typing. Results:There were 16 biochemical types of Y. pestis in the natural foci of plague in China, and each biochemical type showed obvious regional distribution in each foci. Most strains were positive for ass hide glue glycolysis (89.79%, 1 961/2 184), maltose (80.13%, 1 750/2 184), glycerol (94.23%, 2 058/2 184), and denitrification (82.78%, 1 808/2 184), and negative for rhamnose (88.78%, 1 939/2 184) and melibiose (85.62%, 1 870/2 184). Virulence factor test results showed that 99.95% (2 183/2 184) of Y. pestis were F1 positive; 99.73% (2 178/2 184) of Y. pestis can produce Pst Ⅰ; 73.31% (1 601/2 184) of Y. pestis were VWa positive and 26.69% (583/2 184) were VWa negative; Pgm positive strains accounted for 72.62% (1 586/2 184), Pgm negative strains accounted for 21.52% (470/2 184), and Pgm mixed type strains accounted for 5.86% (128/2 184). According to DFR typing results, there were 52 genotypes in 2 184 strains of Y. pestis, of which 19 were major genotypes and 33 were minor genotypes. CRISPR typing revealed 16 major genotypes, of which 7 were newly discovered. Conclusion:The phenotypes and genotypes of Y. pestis in various natural foci of plague in China are diverse and have geographical distribution characteristics.
