Construction, identification and expression of recombinant Schistosoma japonicum vaccine mediated by Enterococcus faecalis
10.3760/cma.j.cn231583-20220226-00047
- VernacularTitle:粪肠球菌介导的日本血吸虫重组疫苗的构建、鉴定及表达
- Author:
Wengui LI
1
;
Xingkun OU
;
Ailin HE
Author Information
1. 重庆医科大学附属第一医院传染病寄生虫病研究所，重庆 400016
- Keywords:
Enterococcus faecalis;
Schistosoma japonicum;
Recombinant vaccine;
Sj26GST-GST fusion protein
- From:
Chinese Journal of Endemiology
2023;42(3):173-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a recombinant vaccine of Schistosoma japonicum (Sj) mediated by Enterococcus faecalis (Efs, rEfs-Sj26GST vaccine), and to study the expression of Sj26GST-GST fusion protein in the recombinant vaccine. Methods:The recombinant plasmid pGEX-Sj26GST was transformed into the susceptible strain Efs ATCC47077 by electroporation to construct rEfs-Sj26GST vaccine, and the plasmid was extracted for PCR identification. After induction of expression with isopropyl-beta-D-thiogalactopyranoside (IPTG), the products were analyzed and identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot.Results:After PCR identification, a 676 bp fragment was amplified, which was consistent with the length of Sj26GST amplification fragment. SDS-PAGE analysis showed that the relative molecular mass was 52 × 10 3, which was consistent with the band of Sj26GST-GST fusion protein. Western blot results showed that the Sj26GST-GST fusion protein expressed by rEfs-Sj26GST vaccine could be specifically recognized by the serum of Sj infected patients. Conclusion:The rEfs-Sj26GST vaccine is successfully constructed, and the Sj26GST-GST fusion protein expressed by recombinant vaccine can be specifically recognized by the serum of Sj infected patients.
