Multi-locus sequence typing of human Brucella isolated in Sichuan Province from 2014 to 2020
10.3760/cma.j.cn231583-20211214-00405
- VernacularTitle:2014 - 2020年四川省人间布鲁氏菌病分离菌株多位点序列分型研究
- Author:
Wenbo LI
1
;
Shicheng ZHAO
;
Dan GAO
;
Chunhua LUO
;
Linzi ZENG
;
Hongyu LIAO
;
Teng QI
;
Lixin XU
Author Information
1. 四川省疾病预防控制中心微生物检验所，成都 610041
- Keywords:
Brucella;
Multi-locus sequence typing;
Molecular typing
- From:
Chinese Journal of Endemiology
2023;42(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To learn about the genotyping of human Brucella isolated from Sichuan Province. Methods:BCSP31-PCR and AMOS-PCR were used to identify the genus and biotype of the 66 strains isolated from confirmed human brucellosis cases in Sichuan Province from 2014 to 2020, respectively. The isolated strains were genotyped by multi-locus sequence typing (MLST)-9. The sequence type (ST) was compared trough the online MLST database. A minimum spanning tree (MST) was constructed to cluster the newly discovered and known ST using the BioNumerics software version 7.6.Results:The 66 strains isolated from human cases of brucellosis in Sichuan Province from 2014 to 2020 were Brucella, and 65 of them were Brucella melitensis while one strain was Brucella abortus. The MLST method identified three known STs (ST-8, ST-39 and ST-2) and one newly type (ST-101). Among them, ST-8 was the main ST in Sichuan Province (90.91%, 60/66), another 4 strains of Brucella melitensis were ST-39, and 1 strain of Brucella abortus was ST-2. The newly type ST-101 was isolated from Leshan City in 2019, belonging to the Brucella melitensis and closely related to the evolution of ST-8. Conclusion:Brucella melitensis is the main epidemic Brucella strain in Sichuan Province, ST-8 is predominant genotype, with a small amount of ST-39, ST-101 and ST-2.
