Serotonin 1A Receptor Agonist(8 OH DPAT) Reduces Tissue Lesion Volume in the Rat Middle Cerebral Artery Occlusion Model of Ischemia.
- Author:
Do Heum YOON
1
;
Young Soo KIM
;
Wise YOUNG
Author Information
1. Department of Neurosurgery, College of Medicine, Yonsei University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Middle cerebral artery occlusion;
Serotonin;
8 OH DPAT;
Brain ischemia;
Lesion volume
- MeSH:
Absorption;
Animals;
Brain Ischemia;
Cell Size;
Infarction, Middle Cerebral Artery*;
Infusions, Intravenous;
Ischemia*;
Middle Cerebral Artery*;
Potassium;
Rats*;
Receptor, Serotonin, 5-HT1A*;
Serotonin*;
Sodium;
Spectrum Analysis
- From:Journal of Korean Neurosurgical Society
1996;25(7):1352-1363
- CountryRepublic of Korea
- Language:English
-
Abstract:
We studied the effectiveness of serotonin 1a agonist(8 OH DPAT) on the 24 hour ionic lesion volume produced by permanent occlusion of the middle cerebral artery(MCAo) in rats. A 4-hour intravenous infusion of 30 micro mg/kg/hr of 8 OH DPAT were given starting at 10 minutes after occlusion. Tissue concentrations of Na, K, and water at infarct and peri-infarct zone were measured by atomic absorption spectroscopy and by wet-dry weight measurements 24 hours after occlusion. Compared with vehicle treatment, 8 OH DPAT treatment reduced tissue water accumulation by 10% and 55% in the frontopyriform cortex(L1) and frontoparietal cortex(L2), sodium accumulation by 20% at L1 and 47% at L2, potassium loss by 24% at L1 and 44% at L2, cell volume fraction loss by 24% at L1 and 47% at L2. Finally the treatment reduced overall lesion volume by about 37%. All these changes were statistically significant at p<0.05. Our findings suggest strongly that 8 OH DPAT is neuroprotective in the rat MCAo model of focal cerebral ischemia.
