DNA barcoding in herbal medicine:Retrospective and prospective
- Author:
Shilin CHEN
1
;
Xianmei YIN
;
Jianping HAN
;
Wei SUN
;
Hui YAO
;
Jingyuan SONG
;
Xiwen LI
Author Information
1. Institute of Herbgenomics,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China;Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700,China
- Keywords:
Herbal identification;
Super-barcode;
Mini-barcode;
Meta-barcoding;
Cutting-edge barcoding
- From:
Journal of Pharmaceutical Analysis
2023;13(5):431-441
- CountryChina
- Language:Chinese
-
Abstract:
DNA barcoding has been widely used for herb identification in recent decades,enabling safety and innovation in the field of herbal medicine.In this article,we summarize recent progress in DNA bar-coding for herbal medicine to provide ideas for the further development and application of this tech-nology.Most importantly,the standard DNA barcode has been extended in two ways.First,while conventional DNA barcodes have been widely promoted for their versatility in the identification of fresh or well-preserved samples,super-barcodes based on plastid genomes have rapidly developed and have shown advantages in species identification at low taxonomic levels.Second,mini-barcodes are attractive because they perform better in cases of degraded DNA from herbal materials.In addition,some mo-lecular techniques,such as high-throughput sequencing and isothermal amplification,are combined with DNA barcodes for species identification,which has expanded the applications of herb identification based on DNA barcoding and brought about the post-DNA-barcoding era.Furthermore,standard and high-species coverage DNA barcode reference libraries have been constructed to provide reference se-quences for species identification,which increases the accuracy and credibility of species discrimination based on DNA barcodes.In summary,DNA barcoding should play a key role in the quality control of traditional herbal medicine and in the international herb trade.
