Rapid authentication of different herbal medicines by heating online extraction electrospray ionization mass spectrometry
- Author:
Zidong QIU
1
;
Chaofa WEI
;
Xiang LI
;
Changjiangsheng LAI
;
Zhilai ZHAN
;
Yan JIN
;
Li ZHOU
;
Qingxiu HAO
;
Jian YANG
;
Shuanglong WANG
;
Liping KANG
;
Luqi HUANG
Author Information
1. State Key Laboratory ofDao-di Herbs,National Resource Centre for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700,China
- Keywords:
Heating online extraction electrospray ionization mass spectrometry;
Rapid authentication;
Traditional Chinese medicine
- From:
Journal of Pharmaceutical Analysis
2023;13(3):296-304
- CountryChina
- Language:Chinese
-
Abstract:
The rapid and accurate authentication of traditional Chinese medicines(TCMs)has always been a key scientific and technical problem in the field of pharmaceutical analysis.Herein,a novel heating online extraction electrospray ionization mass spectrometry(H-oEESI-MS)was developed for the rapid and direct analysis of extremely complex substances without the requirement for any sample pretreatment or pre-separation steps.The overall molecular profile and fragment structure features of various herbal medicines could be completely captured within 10-15 s,with minimal sample(＜0.5 mg)and solvent consumption(＜20 μL for one sample).Furthermore,a rapid differentiation and authentication strategy for TCMs based on H-oEESI-MS was proposed,including metabolic profile characterization,characteristic marker screening and identification,and multivariate statistical analysis model validation.In an analysis of 52 batches of seven types of Aconitum medicinal materials,20 and 21 key compounds were screened out as the characteristic markers of raw and processed Aconitum herbal medicines,respectively,and the possible structures of all the characteristic markers were comprehensively identified based on Com-pound Discoverer databases.Finally,multivariate statistical analysis showed that all the different types of herbal medicines were well differentiated and identified(R2X＞0.87,R2Y＞0.91,and Q2＞0.72),which further verified the feasibility and reliability of this comprehensive strategy for the rapid authentication of different TCMs based on H-oEESI-MS.In summary,this rapid authentication strategy realized the ultra-high-throughput,low-cost,and standardized detection of various complex TCMs for the first time,thereby demonstrating wide applicability and value for the development of quality standards for TCMs.
