A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography
- Author:
Yikun LIU
1
,
2
;
Ning HE
;
Yingfang LU
;
Weiqiang LI
;
Xin HE
;
Zhentao LI
;
Zilin CHEN
Author Information
1. Department of Orthopedics Trauma and Microsurgery,Zhongnan Hospital of Wuhan University,School of Pharmaceutical Sciences,Wuhan University,Wuhan,430072,China
2. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery,Ministry of Education,Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals,Wuhan,430071,China
- Keywords:
Sodium p-styrene sulfona;
Stationary phase;
Monolithic column;
Capillary electrochromatography;
Bifunctional interaction
- From:
Journal of Pharmaceutical Analysis
2023;13(2):209-215
- CountryChina
- Language:Chinese
-
Abstract:
Here,a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM)with reversed-phase/hydrophilic interaction liquid chromatography(RPLC/HILIC)bifunctional separation mode was success-fully prepared for capillary electrochromatography by the in situ polymerization of sodium p-styrene sulfonate(VBS)with cross-linkers 3-(acryloyloxy)-2-hydroxypropyl methacrylate(AHM)and 1,3,5-triacryloylhexahydro-1,3,5-triazine(TAT).The preparation conditions of the monolith were optimized.The morphology and formation of the poly(VBS-co-TAT-co-AHM)monolith were confirmed by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FT-IR).The separation perfor-mances of the monolith were evaluated systematically.It should be noted that the incorporation of VBS functional monomer can provide π-π interactions,hydrophilic interactions,and ion-exchange in-teractions.Hence,the prepared poly(VBS-co-TAT-co-AHM)monolith can achieve efficient separation of thiourea compounds,benzene series,phenol compounds,aniline compounds and sulfonamides in RPLC or HILIC separation mode.The largest theoretical plate number for N,N'-dimethylthiourea reached 1.7×105 plates/m.In addition,the poly(VBS-co-TAT-co-AHM)monolithic column showed excellent reproducibility and stability.This novel monolithic column has great application value and potential in capillary electrochromatography(CEC).
