Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography
- Author:
Qiqin WANG
1
;
Lingjue SUN
;
Huihui WU
;
Ning DENG
;
Xianglong ZHAO
;
Jingwei ZHOU
;
Tingting ZHANG
;
Hai HAN
;
Zhengjin JIANG
Author Information
1. Institute of Pharmaceutical Analysis,College of Pharmacy,Jinan University,Guangzhou,510632,China
- Keywords:
Zwitterionic monolith;
Sulfobetaine;
Photo-initiated copolymerization;
Hydrophilic interaction chromatography;
Complex sample
- From:
Journal of Pharmaceutical Analysis
2022;12(5):783-790
- CountryChina
- Language:Chinese
-
Abstract:
Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction chromatography.In this study,a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-l-propanesulfonate(SBVI)with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic system.Notably,the duration for the preparation of this novel monolith was as little as 5 min,which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated copolymerization.Moreover,these monoliths showed good morphology,permeability,porosity(62.4%),mechanical strength(over 15 MPa),column efficiency(51,230 plates/m),and reproducibility(relative standard deviations for all analytes were lower than 4.6%).Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based monolith.In particular,the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein adsorption.These excellent features seem to favor its application in bioanalysis.Therefore,the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex samples.In this study,we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.