Effect of epidermal growth factor on growth of human renal cell carcinoma and transitional cell carcinoma cell lines.
- Author:
Eun Sik LEE
1
;
Hyeon Hoe KIM
;
Chong Wook LEE
Author Information
1. Department of Urology, Seoul National University, College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cell lines;
EGF receptor;
Cell growth
- MeSH:
Carcinoma, Renal Cell*;
Carcinoma, Transitional Cell*;
Cell Line*;
Epidermal Growth Factor*;
Humans*;
Receptor, Epidermal Growth Factor
- From:Korean Journal of Urology
1993;34(2):212-219
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We evaluated the relationship between content of epidermal growth factor receptor (EGFR) and responsiveness to epidermal growth factor (EGF) in human renal cell carcinoma (RCC) and transitional cell carcinoma (TCC) cell lines. EGFR was measured by radioisotope ligand binding assay using I-EGF. Three renal cell carcinoma cell lines (Caki-1. Caki-2, A-498) and 4 transitional cell carcinoma cell lines (T-24. J-82. HT-1197. HT-1376) were used. Growth of cell lines was tested in serum free RPMI 1640 media with or without various concentration of EGF by MTT assay. The content of EGFR was far higher in HT-1197than the other cell lines. EGFR contents were higher in RCC than in TCC cell lines except HT-1197. All RCC and TCC cell lines did not grow in serum free RPMI 1640 media. In EGF supplemented condition, HT-1197 and 3 RCC cell lines which showed high content of EGFR were stimulated to grow in a dose related fashion, but T-24. J-82 and HT-1376 cell lines which showed low content of EGFR were not. Growth rates of RCC and TCC cell lines were closely related with the content of EGFR under influence of EGF.
