Highly Protective Association of MMP-2-1306C/T Promoter Polymorphism With Asthma in a North Indian Population: A Pilot Study.
10.4168/aair.2014.6.3.234
- Author:
Niti BIRBIAN
1
;
Jagtar SINGH
;
Surinder Kumar JINDAL
Author Information
1. Department of Biotechnology, Panjab University, Chandigarh, India. jagtar72@gmail.com
- Publication Type:Original Article
- Keywords:
Asthma;
collagenase;
matrix metalloproteinase-2 (MMP-2);
polymorphism
- MeSH:
Adult;
Alleles;
Arm;
Asthma*;
Case-Control Studies;
Collagen Type IV;
Collagenases;
Genotype;
Humans;
India;
Matrix Metalloproteinase 2;
Pilot Projects*;
Polymerase Chain Reaction;
Polymorphism, Single Nucleotide
- From:Allergy, Asthma & Immunology Research
2014;6(3):234-241
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Asthma is the most prevalent disease in India according to the national survey conducted by NFHS 2 in 1998-1999. Matrix metalloproteinase-2 (MMP-2), a collagenase encoded by the MMP-2 gene, degrades the type IV collagen and is responsible for inflammatory responses. This is a pilot study evaluating the role of MMP-2 -1306C/T promoter single nucleotide polymorphism (SNP) in asthma pathogenesis. METHODS: A case-control study was performed with a total of 824 adult subjects, including 410 adult asthmatics and 414 healthy controls from regions of North India. The MMP-2 -1306C/T polymorphism was genotyped by the Tetra-Primer Amplification Refractory Mutation System Polymerase Chain Reaction (Tetra-Primer ARMS PCR). RESULTS: Statistical analysis of the results for the MMP-2 -1306C/T polymorphism revealed an extremely protective role of the mutant T allele in asthma pathogenesis with OR=0.45, 95% CI (0.35-0.58) and P=0.000. The heterozygous CT genotype also conferred protection from asthma with OR=0.37, 95% CI (0.27-0.51) and P=0.000. The homozygous TT genotype was also significantly associated with asthma with OR=0.35, 95% CI (0.16-0.72) and P=0.002. Moreover, the polymorphism was significantly associated with all the phenotypic traits of the disease. CONCLUSION: The MMP-2 -1306C/T promoter polymorphism confers significant protection from asthma in the studied North Indian population
