Effect of trichostatin A on lipopolysaccharide-induced injury and apoptosis of human microvascular endothelial cell
10.3760/cma.j.issn.1673-4912.2023.03.009
- VernacularTitle:曲古霉素A对脂多糖诱导的人微血管内皮细胞损伤和凋亡的影响
- Author:
Fengsheng WU
1
;
Yu LIU
;
Haiying LIU
;
Chunyuan WU
;
Jieming YU
;
Hongxia ZHAO
Author Information
1. 南方医科大学附属深圳妇幼保健院PICU 518028
- Keywords:
Histone deacetylase inhibitors;
Trichostatin A;
Sepsis;
Cellular damage;
Apoptosis
- From:
Chinese Pediatric Emergency Medicine
2023;30(3):203-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of histone deacetylase inhibitor trichostatin A(TSA) on the lipopolysaccharide(LPS)-induced injury and apoptosis of human microvascular endothelial cell(HMEC).Methods:HMECs were used as research cells to establish LPS-induced septic cell model, which were divided into three groups according to different treatments: control group (150 μL of phosphate buffer), LPS group (150 μL of 5 μg/mL LPS), LPS+ TSA group (150 μL of 5 μg/mL LPS and 500 μg/L TSA). After cells of each group were cultured for 24 h and 48 h, the concentration of lactate dehydrogenase(LDH)in the culture supernatant was detected by enzyme-linked immunosorbent assay and the apoptosis rate of HMECs was detected by Annexin V-FTTC/PI staining, then comparison between different groups were made.Results:Compared with the control group, LDH concentration in LPS group increased significantly at 24 h[(4.67±1.27) ng/L vs. (11.57±0.83) ng/L ] and 48 h[(7.93±0.80) ng/L vs. (12.72±0.89) ng/L ]; Compared with LPS group, LDH concentration in LPS + TSA group decreased significantly at 24 h[(6.01±0.29) ng/L ] and 48 h[(5.96±0.27) ng/L ], and the differences were statistically significant ( P<0.05). Compared with the control group, the apoptosis rates of HMEC cells in LPS group were significantly higher at 24 h[(0.92±0.89)% vs. (1.66±0.09)% ] and 48 h[(1.09±0.14)% vs. (5.01±0.16)%]; Compared with LPS group, the apoptosis rate of HMEC cells in LPS + TSA group significantly decreased at 24 h[(1.36±0.01)% ] and 48 h[(4.19±0.23)% ], the differences were statistically significant ( P<0.05). Conclusion:TSA has the protective effect of reducing cell injury and apoptosis in sepsis.
