Evaluation of multiplex PCR for viral central nervous system infection in children
10.3760/cma.j.issn.1673-4912.2022.12.013
- VernacularTitle:多重病原体核酸分子检测在儿童病毒性中枢神经系统感染的诊断效果评价
- Author:
Yue ZHU
1
;
Qiaozhen ZHANG
;
Bailu DU
;
Ruike ZHAO
;
Yue TAO
;
Xiaoxia DENG
;
Qing CAO
Author Information
1. 上海交通大学附属医学院附属上海儿童医学中心感染科 200127
- Keywords:
Viral encephalitis;
Rapid diagnosis;
Molecular diagnosis;
Film Array meningitis/encephalitis panel
- From:
Chinese Pediatric Emergency Medicine
2022;29(12):998-1002
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the clinical significance of molecular detection testing multiple pathogens in children with viral central nervous system infections.Methods:We retrospectively included 176 children who were suspected with central nervous system infection at Shanghai Children′s Medical Center from January 2017 to May 2021.Film Array Meningitis/Encephalitis Panel(FA-M/E) was used to test cerebrospinal fluid samples of these children.The results were analyzed compared with clinical symptoms and cerebrospinal fluid indices.Results:There were 34 samples with positive FA-M/E virus detection(19.32%, 34/176). Among the 34 samples, enterovirus was the most common pathogen(27 cases, 79.41%). In different combinations, the sensitivity and positive predictive value were all less than 90%.The median time for antiviral drugs used in FA-M/E virus-positive and negative children was 4.5(0, 8.5)d and 2.6(0, 2.0)d, respectively.The difference was statistically significant( P<0.05). Conclusion:Molecular tests of multiple pathogens can quickly and sensitively detect pathogens.It can improve the efficacy of clinical diagnosis of viral central nervous system infection.
